Modeling structure and flexibility of Candida antarctica lipase B in organic solvents
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چکیده
منابع مشابه
Rational redesign of Candida antarctica lipase B
This thesis describes the use of rational redesign to modify the properties of the enzyme Candida antarctica lipase B. Through carefully selected single-point mutations, we were able to introduce substrate-assisted catalysis and to alter the reaction specificity. Other single-point mutations afforded variants with greatly changed substrate selectivity and enantioselectivity. Mutation of the cat...
متن کاملFunctional expression of Candida antarctica lipase B in Eschericha coli.
Candida antarctica lipase B (CalB) is an important catalyst in bio-organic synthesis. To optimize its performance, either the reaction medium is changed or the lipase itself is modified. In the latter case, mutants are generated in Eschericha coli and subsequently expressed in fungal hosts for their characterization. Here we present the functional expression of CalB in the periplasm of E. coli....
متن کاملEffect of Water Clustering on the Activity of Candida antarctica Lipase B in Organic Medium
The effect of initial water activity of MTBE (methyl tert-butyl ether) medium on CALB (Candida antarctica lipase B) catalyzed esterification reaction is investigated using experimental methods and classical molecular dynamics (MD) simulations. The experimental kinetic studies show that the initial reaction rate of CALB-catalyzed esterification reaction between butyric acid and ethanol decreases...
متن کاملModelling of Enantioselectivity in Candida antarctica Lipase B
Enzyme selectivity towards a particular enantiomeric configuration of substrate is necessarily governed by chemical and spatial relationships between the substrate and the enzyme binding cavity. In the case of Candida antarctica lipase B experimental evidence shows that enantioselectivity of the enzyme towards the alcohol moiety 3-methyl-2-butanol varies significantly with respect to the chain ...
متن کاملA novel self-activation mechanism of Candida antarctica lipase B.
Candida antarctica lipase B (CalB), resembling many other lipases structure-wise, contains a flexible lid that undergoes a surprisingly large conformational change when catalyzing hydrophobic substrates (e.g. triglycerides). Despite extensive and important applications in industry, it is so far still elusive whether CalB can be activated on a hydrophobic surface, like other lipases. From large-...
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ژورنال
عنوان ژورنال: BMC Structural Biology
سال: 2008
ISSN: 1472-6807
DOI: 10.1186/1472-6807-8-9